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Laboratory of cell and tissue cultures
BD FACSAria optics configurations
| Laser |
Mirror
(nm) |
Filter
(nm) |
Standard Fluorochrome
(Em. max., nm) |
Alternative Fluorochrome
(Em. max., nm) |
| Blue (488 nm) |
735 |
780/60 |
PE-Cy7 (767) |
|
| 655 |
695/40 |
PerCP-Cy5.5 (710) |
PE-Cy5 (670), PE-Cy5.5 (710), PerCP (675), Tri-Color, TruRed, DRAQ5, 7-AAD (647), SNARF-1 (>650), Acridin Orange-RNA (650), PI |
| 610 |
616/23 |
PE-TexasRed |
ECD, EMA, Alexa 610-PE, Red 613, Ethidium Bromide (620) |
| 556 |
585/42 |
PE (578)
|
DsRed, Vybrant Orange, PKH 26 (567), BRSE (568), RFP, Cy3 (566) Sytox orange (570) |
| 502 |
530/30 |
FITC (517) |
Alexa 488 (520), GFP, EGFP (508), YFP (527), CFSE (525), CFDA-SE (517), CMFDA (520), DCF, PKH 67 (502), PKH2 (504), Oregon Green 488 (514), Vybrant Green, SE (518), 5'6Carboxyfluorescein, S65L (510), S65T (511), ZsGreen1 (493), Calcein AM (517), Cy2 (506), Acridin Orange-DNA (526), Sytox green (523) Thiazole orange (530) TO-PRO1 (531), TOTO (533), YO-PRO1 (506), YOYO-1 (508) |
| |
488/10 |
side scatter |
|
| Red (633 nm) |
735 |
780/60 |
APC-Cy7 (755)
|
PharRed, Alexa 750 |
| |
660/20 |
APC (660) |
Alexa 633 (650), Alexa 647 (666), Red C, Cy5 (666), DDAO-SE (659), CellVue Plum (671), CellVue Claret (675) TO-PRO1 (657), TOTO-3 (660) |
| Violet (407 nm) |
502 |
530/30 |
Alexa 430 |
Cascade Yellow (550), AmCyan, Vex, Live Dead Aqua, QD525 |
| |
450/40 |
DAPI (461),
Hoechst 34580 (440),
Cascade Blue (420),
Pacific Blue (455),
Alexa 405 (421)
|
Hydrazide, Live/Dead fixable Violet stain, SYTOX Blue Dead Cell stain, Vybrant DyeCycle Violet stain, PO-PRO-1, ECFP, Calcein Violet, AM, CellVue Lavender (461), Hoechst 33342 (483) Sytox blue (470) |
Notes:
FITC – fluorescein isothiocyanat
PE – phycoerythrin
GFP – green fluorescent protein
YFP – yellow fluorescent protein
PI – propidium iodide
APC – allophycocyanin
PerCP – peridinin chlorophyll protein
CFSE – carboxyfluorescein succinimidyl ester
7-AAD – 7-aminoactinomycin D
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