Laboratory of Cell and Tissue Cultures

Vyshgorodska street 67, Kyiv 04114, Ukraine
e-mail: labcellculture[a]gmail.com

Alina USTYMENKO  - Head of Laboratory, Ph.D. (Biology)

Galyna SKIBO  - Principal Researcher, Professor, Sc.D. (Medicine)

Oleg TSUPYKOV  -  Senior Researcher, Sc.D. (Medicine)

Oksana RYBACHUK - Researcher, Ph.D. (Biology)

Pavlo KLYMENKO  - Junior Researcher, Ph.D. (Biology)

Galyna SHMATKO - Technician

Main research directions

- study of neural stem cells at experimental ischemic stroke in mice in vivo. More...

- study of regenerative potential of different type of stem cells at brain ischemia in vitro.  More...

- study of regenerative potential of adipose-derived stem cells at critical limb ischemia.

- study of regenerative potential of stem cells at heart ischemia.

Methods 

- multiparametric cell phenotyping and sorting by flow cytometry. More...

- cell and tissue phenotyping using immunocyto- and immunohistochemistry.

- stem cell culture and directed differentiation. More...

- mouse embryo culture and micromanipulation. More... 

- modelling of injury of different tissues on laboratory mice.

Main publications

Articles

• Kyryk V, Parkhomenko O. Potential prognostic markers of cellular senescence in age-associated cardiovascular pathology. Fiziolohichnyi zhurnal. 2025; 71(2):93-105. https://doi.org/10.15407/fz71.02.093
• Shevchuk OI, Korcheva VV, Moskalenko NS, Kyryk V, Kot K, Krasnienkov D. Application of Decellularization Methods for Scaffold Production: Advantages, Disadvantages, Biosafety and Modifications. Front. Bioeng. Biotechnol. 2025; 13. https://doi.org/10.3389/fbioe.2025.1621641 
• Лабунець ІФ, Клименко ПП, Кирик ВМ, Довбинчук ТВ, Пантелеймонова ТМ. Морфологічна характеристика міокарда на фоні зміни вмісту імунних клітин в головному мозку при змодельованому паркінсонізмі у мишей різних ліній. Український кардіологічний журнал. 2025. 32(4): 50-58. https://doi.org/10.31928/2664-4479-2025.4.5058
• Kyryk V, Ustymenko A, Tsupykov O. The effect of ageing on the properties of adipose-derived stem cells. Literature review. Aging and Longevity. 2023; 4(3): 74-85. https://doi.org/10.47855/jal9020-2023-3-2
• Labunets I, Toporova O, Panteleymonova T, Dovbynchuk T, Kyryk V, Kashchuk O, Kordium V. Comparative effects of human umbilical cord-derived mesenchymal stromal cells and their extracellular vesicles in a mouse model of parkinsonism. Cell Organ Transpl. 2025; 13(1):46-53 (e2025131176). https://doi.org/10.22494/cot.v13i1.176 
• Holiuk Ye., Birsa R, Bukreieva T, Nemtinov P, Kyryk V, Ustymenko A, Mazevych V, Sokolov M, Lobyntseva G, Shablii V. Effectiveness and safety of multiple injections of human placenta-derived MSCs for knee osteoarthritis: nonrandomized phase I trial. BMC Musculoskeletal Disorders. 2025; 26(418). https://doi.org/10.1186/s12891-025-08664-2 

• Kyryk V. Cell therapy for cardiac regeneration: key aspects. Cell Organ Transpl. 2024; 12(1):4-21. DOI: https://doi.org/10.22494/cot.v12i1.160

• Labunets I, Ustymenko A, Panteleymonova T, Rodnichenko A. Colony-forming potential of murine bone marrow-derived progenitor cells in experimental neurodegenerative pathology and under the impact of melatonin in vivo or in vitro. Cell Organ Transpl. 2024; 12(1):52-58. DOI: https://doi.org/10.22494/cot.v12i1.165 
• Ustymenko A., Tsupykov O., Kyryk V. Prospects for cytokinesis-block micronucleus cytome assay in the comprehensive evaluation of cellular senescence markers. Cell Organ Transpl. 2024; 12(2):e2024122172. DOI: https://doi.org/10.22494/cot.v12i2.172
• Kyryk V, Ustymenko A, Klymenko P, Tsupykov O. Development and validation of a lipopolysaccharide-induced myocardial inflammation model in mice for preclinical research on the regenerative potential of stem cells. Anti-Aging Eastern Europe. 2024; 3(4):221-232. DOI:https://doi.org/10.56543/aaeeu.2024.3.4.09
• Bukreieva T, Svitina H, Nikulina V, Vega A, Chybisov O, Shablii I, Ustymenko A, Nemtinov P, Lobyntseva G, Skrypkina I, Shablii V. Treatment of Acute Respiratory Distress Syndrome Caused by COVID-19 with Human Umbilical Cord Mesenchymal Stem Cells. Int J Mol Sci. 2023 Feb 23;24(5):4435. Available from: https://doi.org/10.3390/ijms24054435
• Labunets I, Panteleymonova T, Kyryk V, Toporova O, Litoschenko Z. Effects of human umbilical cord-derived multipotent mesenchymal stromal cells transplantation in mice of different strains with an experimental model of parkinsonism. Cell Organ Transpl. 2023; 11(2):96-103. https://doi.org/10.22494/cot.v11i2.155
• Bukreieva T, Svitina H, Nikulina V, Vega A, Chybisov O, Shablii I, Ustymenko A, Nemtinov P, Lobyntseva G, Skrypkina I, Shablii V. Treatment of Acute Respiratory Distress Syndrome Caused by COVID-19 with Human Umbilical Cord Mesenchymal Stem Cells. Int J Mol Sci. 2023 Feb 23;24(5):4435. Available from: https://doi.org/10.3390/ijms24054435
  
• Bukreieva T, Kyryk V, Nikulina V, Svitina H, Vega A, Chybisov O, Shablii I, Mankovska O, Lobyntseva G, Nemtinov P, Skrypkina I, Shablii V. Dynamic changes in radiological parameters, immune cells, selected miRNAs, and cytokine levels in peripheral blood of patients with severe COVID‑19. Biomed Rep. 2023 Mar 21;18(5):33. https://doi.org/10.3892/br.2023.1615
• Shatilo VB, Antoniuk-Shcheglova IA, Bondarenko OV, Naskalova SS, Pisaruk АV, Mekhova LV, Ustymenko AM. Markers of psycho-emotional stress in women of the Kyiv region the Russian-Ukrainian war. Fiziol. Zh. 2023; 69(4): 3-10. Available from: https://doi.org/10.15407/fz69.04.003
• Kyryk V, Tsupykov O, Ustymenko A, Smozhanik E, Govbakh I, Butenko G, Skibo G. Age-related ultrastructural changes in spheroids of the adipose-derived multipotent mesenchymal stromal cells from ovariectomized mice. Front Cell Neurosci. 2023 Feb 17;17:1072750. https://doi.org/10.3389/fncel.2023.1072750
• Kyryk V, Ustymenko A, Lutsenko T, Klymenko P, Tsupykov O. Regenerative effects of mouse aortic endothelial cells in a murine model of critical limb ischemia. Cell Organ Transpl. 2022; 10(2):90-96. Available from: https://doi.org/10.22494/cot.v10i2.143
• Ustymenko A, Kyryk V, Butenko G. Morphofunctional characteristics of adipose-derived multipotent mesenchymal stromal cells from CBA/Ca mice of different ages in cell culture in vitro. Cell Organ Transpl. 2022; 10(1):46-51. https://doi.org/10.22494/cot.v10i1.137 
• Ivanishchev V, Ustymenko A, Butenko G. Morphometric analysis of skin in ovariectomized mice of different ages. Cell Organ Transpl. 2022; 10(1):54-60. DOI: https://doi.org/10.22494/cot.v10i1.138
• Labunets I, Utko N, Panteleymonova T, Kyryk V, Kharkevych Yu, Rodnichenko A, Litoshenko Z, Butenko G. Effects of transplanted adipose-derived multipotent mesenchymal stromal cells from mice of different age or from aging donors in combination with melatonin at experimental parkinsonism. Cell Organ Transpl. 2022; 10(1):18-24. https://doi.org/10.22494/cot.v10i1.134
• Kyryk V, Kuchuk O, Klymenko P. Regenerative effects of mouse adipose-derived multipotent stromal cells in a micromass graft for the treatment of bone injury model. Anti-Aging Eastern Europe. 2022; l(1):75-86. https://doi.org/10.56543/aaeeu.2022.1.1.11
• Kalmukova O, Kyryk V, Dzerzhynsky M. Circadian rhythms and personalized strategies for anti-aging therapies. Anti-Aging Eastern Europe. 2022; 1(1):12-21. https://doi.org/10.56543/aaeeu.2022.1.1.03
• Kyryk V, Ustymenko A. Isolation and phenotyping of cardiac-derived progenitor cells from neonatal mice. Cell Organ Transpl. 2021; 9(2):126-133. https://doi.org/10.22494/cot.v9i2.125
• Govbakh I, Kyryk V, Ustymenko A, Rubtsov V, Tsupykov O, Bulgakova N, Zavodovskiy D, Sokolowska I, Maznychenko A. Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy. Int. J. Mol. Sci. 2021; 22(21):12026. https://doi.org/10.3390/ijms222112026
• I. Govbakh, K. Smozhanik, M. Patseva, V. Rubtsov, A. Ustymenko, V. Kyryk, O. Tsupykov Ultrastructural analysis of sciatic nerve in mice with peripheral neuropathy after transplantation of adipose-derived multipotent mesenchymal stromal cells. Fiziol. Zh. 2021; 67(3): 17-26. DOI: https://doi.org/10.15407/fz67.03.017
• Lisyany M, Govbakh I, Belska L, Tsupykov O, Palamarova A. The effects of the conditioned medium of fetal neural cell culture on the apoptosis in the rat brain after traumatic brain injury. Cell Organ Transpl. 2021; 9(1):30-34. https://doi.org/10.22494/cot.v9i1.119
• Rubtsov V, Govbach I, Ustymenko A, Kyryk V, Tsupykov O. The effects of adipose-derived multipotent mesenchymal stromal cells transplantation on locomotor activity and function of the sciatic nerve in mice with peripheral neuropathy. Cell and Organ Transplantology. 2020; 8(2):159-165. https://doi.org/10.22494/cot.v8i2.111
• Palma-Tortosa S, Tornero D, Grønning Hansen M, Monni E, Hajy M, Kartsivadze S, Aktay S, Tsupykov O, Parmar M, Deisseroth K, Skibo G, Lindvall O, Kokaia Z. Activity in grafted human iPS cell-derived cortical neurons integrated in stroke-injured rat brain regulates motor behavior. PNAS USA. 2020;21;117(16):9094-9100. https://doi.org/10.1073/pnas.2000690117.
• Grønning Hansen M, Laterza C, Palma-Tortosa S, Kvist G, Monni E, Tsupykov O, Tornero D, Uoshima N, Soriano J, Bengzon J, Martino G, Skibo G, Lindvall O, Kokaia Z. Grafted human pluripotent stem cell-derived cortical neurons integrate into adult human cortical neural circuitry. Stem Cells Transl Med. 2020;9(11):1365-1367. https://doi.org/10.1002/sctm.20-0134.
• Ivanishchev V, Ustymenko A, Kyryk V, Butenko G. Comparative morphometric study of adipose tissue in ovariectomized mice of different ages. Cell and Organ Transplantology. 2020; 8(1):64-69. https://doi.org/10.22494/cot.v8i1.108 
• Govbakh IO, Tsupykov OM, Smozhanik EG, Rubtsov VV, Tymchyshin M, Kravchenko SA, Vasylenko DA.  Electron microscopy analysis of sciatic nerve fibers in C57BL/6 Transgenic Mice. Neurophysiology. 2020;52:94–100. https://doi.org/10.1007/s11062-020-09857-2.
• Yatsenko K, Lushnikova I Ustymenko A, Patseva M, Govbakh I, Kyryk V, Tsupykov O. Adipose-Derived Stem Cells Reduce Lipopolysaccharide-Induced Myelin Degradation and Neuroinflammatory Responses of Glial Cells in Mice. J. Pers. Med. 2020, 10(3):66. https://doi.org/10.3390/jpm10030066
• Ustymenko A, Kyryk V, Lutsenko T, Tsupykov O, Butenko G. Morphofunctional properties of adipose-derived multipotent mesenchymal stromal cells in vitro in ovariectomized mice of different ages. Cell and Organ Transplantology. 2019; 7(2):158-167. https://doi.org/10.22494/cot.v7i2.102
• Lykhmus O, Kalashnyk O, Koval L, Voytenko L, Uspenska K, Komisarenko S, Deryabina O, Shuvalova N, Kordium V, Ustymenko A, Kyryk V, Skok M. Mesenchymal Stem Cells or Interleukin-6 Improve Episodic Memory of Mice Lacking α7 Nicotinic Acetylcholine Receptors. Neuroscience. 2019; 413:31-44. https://doi.org/10.1016/j.neuroscience.2019.06.004 
• V Shablii, M Kuchma, H Svitina, I Skrypkina, P Areshkov, V Kyryk, T Bukreieva, V Nikulina, Iu Shablii, G Lobyntseva. High Proliferative Placenta-Derived Multipotent Cells Express Cytokeratin 7 at Low Level. BioMed Research International. Volume 2019; Article ID 2098749, 13 pages. https://doi.org/10.1155/2019/2098749
• Skok M., Lykhmus O., Koval L, Voytenko L., Uspenska K., Komisarenko S., Deryabina O., Shuvalova N., Kordium V., Ustymenko A., Kyryk V. Intravenously injected mesenchymal stem cells penetrate the brain and treat inflammation-induced brain damage and memory impairment in mice. Front. Pharmacol., 2019; 10:355.https://doi.org/10.3389/fphar.2019.00355 
• Kalmukova O. O., Yurchenko A. V., Kyryk V. M. , Nepomnyaschy V. M., Savchuk O. M., Dzerzhynsky M. E. Effects of Melatonin Administration in Different Time Modes on Morphofunctional Indices of the Hypothalamic Serotonergic Neurons in Obese Rats. Neurophysiology. 2018; 50:398-408. https://doi.org/10.1007/s11062-019-09771-2
• Rybachuk, O., Kopach, O., Pivneva, T. and Kyryk, V. (2019). Isolation of Neural Stem Cells from the Embryonic Mouse Hippocampus for in vitro Growth or Engraftment into a Host Tissue. Bio-protocol 9(4): e3165. https://doi.org/10.21769/BioProtoc.3165.
• Golovynska I, Kalmukova O, Svitina HM, Kyryk VM, Shablii VA, Senchylo NV, Ostrovska GV, Dzerzhinskyi M, Stepanov YV, Golovynskyi S, Ohulchanskyy TY, Liu L, Garmanchuk LV, Qu J. Morpho-Functional Characteristics of Bone Marrow Multipotent Mesenchymal Stromal Cells after Activation or Inhibition of Epidermal Growth Factor and Toll-Like Receptors or Treatment with DNA Intercalator Cisplatin. Cytometry Part A. 95A: 24–33, 2019. https://doi.org/10.1002/cyto.a.23593.
• Kyryk V, Kuchuk O, Mamchur A, Ustymenko A, Lutsenko T, Tsupykov O, Yatsenko K, Skibo G, Bilko D, Bilko N. 3D culture of murine adipose-derived multipotent mesenchymal stromal cells in hydrogel based on carbomer 974P. Cell and Organ Transplantology. 2018; 6(2):195-201. https://doi.org/10.22494/cot.v6i2.91.
• Svitina, H., Skrypkina, I., Areshkov, P., Kyryk, V., Bukreieva, T., Klymenko, P., Garmanchuk, L., Lobintseva, G., Shablii, V. Transplantation of placenta‑derived multipotent cells in rats with dimethylhydrazine‑induced colon cancer decreases survival rate. Oncology Letters. 2018 (1899): 5034-5042. https://dx.doi.org/10.3892%2Fol.2018.7996
• Kopach O, Rybachuk O, Krotov V, Kyryk V, Voitenko N, Pivneva T. Maturation of neural stem cells and integration into hippocampal circuits: functional study in post-ischemia in situ. J Cell Sci. 2018 Feb 20;131(4). pii: jcs210989. https://doi.org/10.1242/jcs.210989
• Tsupykov O., Lushnikova I., Ustymenko A., Kyryk V., Nikandrova Y., Patseva M., Yatsenko K., Butenko G., Skibo G. The effects of multipotent mesenchymal stromal cells on mouse brain slices at their co-culture in an in vitro model of periventricular leukomalacia. Fiziologichnyi Zhurnal. 2017; 63(5):3-12
• Tornero D., Tsupykov O., Granmo M., Rodriguez C., Grønning-Hansen M., Thelin J., Smozhanik E., Laterza C., Wattananit S., Ge R., Tatarishvili J., Grealish S., Brüstle O., Skibo G., Parmar M., Schouenborg J., Lindvall O., Kokaia Z. Synaptic inputs from stroke-injured brain to grafted human stem cell-derived neurons activated by sensory stimuli. Brain. 2017; 140(3):692-706. https://doi.org/10.1093/brain/aww347
• Kanemitsu M., Tsupykov O., Potter G., Boitard M., Salmon P., Zgraggen E., Gascon E.,  Skibo G, Dayer A.G.,  Kiss J.Z. EMMPRIN overexpression in SVZ neural progenitor cells increases their migration towards ischemic cortex. Experimental Neurology. 2017; 7(3):457-463. https://doi.org/10.1016/j.expneurol.2017.07.009
• Rybachuk O.A., Lazarenko Y.A., Krotov V.V., Voitenko N.V. Structural/Functional Characteristics of Organotypic Spinal Cord Slices under Conditions of Long-Lasting Culturing. Neurophysiology. 2017; 49(2):162-164. https://doi.org/10.1007/s11062-017-9647-5
• Kalmukova OO, Ustymenko AM, Lutsenko TM, Klymenko PP, Kyryk VM. Morphological and functional characteristics of cell culture derived from the mouse nail unit. Cell and Organ Transplantology. 2017; 5(1):62-66. https://doi.org/10.22494/cot.v5i1.69.
• Tsupykov OM, Lushnikova IV, Ustymenko AM, Kyryk VM, Nikandrova YA, Patseva MA, Yatsenko KV, Butenko GM, Skibo GG. Protective effects of adipose-derived multipotent mesenchymal stromal cells of mice on periventricular leukomalacia model in vitro. Cell and Organ Transplantology. 2017; 5(1):28-32. https://doi.org/10.22494/cot.v5i1.66.
• Svitina, H., Kyryk, V., Skrypkina, І., Kuchma, M., Bukreieva, T., Areshkov, P., Shablii, Y., Denis, Y., Klymenko, P., Garmanchuk, L., Ostapchenko, L., Lobintseva, G., Shablii, V. Placenta‑derived multipotent cells have no effect on the size and number of DMH-induced colon tumors in rats. Experimental and Therapeutic Medicine. 2017; 14 (3): 2135-2147.  https://doi.org/10.3892/etm.2017.4792
• Kalmykova OO. Stem cells in nail unit of mammalians. Cell and Organ Transplantology. 2016; 4(1):138-143. https://doi.org/10.22494/COT.V4I1.1
• Shablii V, Kuchma M, Svitina H, Skrypkina I, Areshkov P, Kyryk V, Shablii Yu, Klymenko P, Lukash L, Lobintseva G. Placenta-derived multipotent cells shared the expression of several trophoblast-related genes. Placenta. 2016; 45:128. https://doi.org/10.1016/j.placenta.2016.06.231
• Tsupykov O., Kanemitsu M., Smozhanik E., Skibo G., Dayer A.G., Kiss J.Z. Relationship of Grafted FGF-2 Overexpressing Neural Stem/Progenitor Cells with the Vasculature in the Cerebral Cortex. Cell Transplant. 2016; 25(7):1359-69. https://doi.org/10.3727/096368916x690421
• Kopach O, Maistrenko A, Lushnikova I, Belan P, Skibo G, Voitenko N. HIF-1α-mediated upregulation of SERCA2b: The endogenous mechanism for alleviating the ischemia-induced intracellular Ca(2+) store dysfunction in CA1 and CA3 hippocampal neurons. Cell Calcium. 2016;59(5):251-61. https://doi.org/10.1016/j.ceca.2016.02.014
• Svitina H, Kalmukova O, Shelest D, Skachkova O, Garmanchuk L, Shablii V. Cellular immune response in rats with 1,2-dimethylhydrazineinduced colon cancer after transplantation of placentaderived multipotent cells. Cell and Organ Transplantology. 2016; 4(1):55-60. https://doi.org/10.22494/COT.V4I1.7
• Tsupykov O, Lushnikova I, Nikandrova E, Yatsenko K, Ustymenko A, Kyryk V, Butenko G, Skibo G. Моделювання перивентрикулярної лейкомаляції на органотиповій культурі зрізів головного мозку миші. Cell and Organ Transplantology. 2016; 4(2):188-193. https://doi.org/10.22494/COT.V4I2.60
• Lutsenko TM. Fetal microchimerism and prenatal diagnosis of genetic disorders. Cell and Organ Transplantology. 2016; 4(1):125-131. https://doi.org/10.22494/COT.V4I1.2
• Tsupykov O., Ustymenko A., Kyryk V., Smozhanik E., Yatsenko K., Butenko G., Skibo G. Ultrastructural study of mouse adipose-derived stromal cells induced towards osteogenic direction. Microsc Res Tech. 2016; 79 (6), 557-564. https://doi.org/10.1002/jemt.22670.
• Ustymenko AM. Cell technologies in treatment of human intervertebral disc hernia: perspectives. Cell and organ transplantology. 2015; 3(2):202-207. https://doi.org/10.22494/COT.V3I2.15
• Rybachuk О. А., Кyryk V. М., Poberezhnyi P. A., Butenko G. M., Pivneva Т. А. Plasticity of bone marrow-derived stroma cells at grafting onto neural tissue after ischemic injury in vitro. Genes&Cells. 2015; 10(1):51-61.
• Tsupykov O. M., Kyryk V. M., Ustymenko A. M., Yatsenko K. V., Butenko G. M., Skybo G. G. Effect of transplantation of adipose-derived multipotent mesenchymal stromal cells on the nervous tissue and behavioral responses in a mouse model of periventricular leukomalacia. Cell and organ transplantology. 2015; 3(1):68-73. https://doi.org/10.22494/COT.v3i1.22
• Kuchma MD, Kyryk VM, Svitina HM, Shablii YuM, Lukash LL, Lobyntseva GS, Shablii VA. Comparative analysis of the hematopoietic progenitor cells from placenta, cord blood, and fetal liver, based on their immunophenotype. BioMed Research International, vol. 2015, Article ID 418752, 16 pages, 2015. https://doi.org/10.1155/2015/418752.  
• Tsupykov O. Ultrastructural analysis of murine hippocampal neural progenitor cells in culture. Microscopy Research and Technique. 2015; 78(2):128-133.
• Kyryk V.M. Multiparametric phenotyping and sorting of murine bone marrow haematopoietic stem cells subpopulations by flow cytometry. Biotechnologia Acta, 2014, vol. 7, No 6, pp. 51-56.
• Mamchur AA. Possibilities for involvement of transformed multipotent mesenchymal stem cell in sarcomogenesis as tumor-initiating cells. Cell and organ transplantology. 2014; 2(2):22-25.
• Kyryk V. M. Short-term migration of transplanted Lin^-Sca-1⁺c-kit⁺ hematopoietic stem cells after hippocampal ischemic injury of mice. Cell and organ transplantology, 2014. Vol. 2, № 2, pp. 148-150.
• Tsupykov ОМ. A protocol for isolation of fetal neural progenitor cells from mouse hippocampus. Cell and organ transplantology. 2014; 2(2):45-47.
• Rybachuk O. A., Pivneva T. A. Contribution of neural stem cells to regeneration of the central nervous system. International Journal of Physiology and Pathophysiology, - 2014, Vol. 5, №1, pp. 83-96.
• Tsupykov O. M., Kyryk V. M., Mamchur A. A., Poberezhnyi P. A., Butenko G. M., Skibo G. G. Transplantation of neural progenitor cells stimulates endogenous neurogenesis in mice after ischemic stroke. Cell and organ transplantology. 2014; 2(1):85-89.
• Rybachuk O. A., Kyryk V. M, Poberezhnyi P. A., Butenko G. M., Skibo G. G., Pivneva T. A. Effect of bone marrow multipotent mesenchymal stromal cells on the neural tissue after ischemic injury in vitro. Cell and organ transplantology. 2014, 2, № 1, p. 74-78.
• O. Tsupykov, V. Kyryk, E. Smozhanik, O. Rybachuk, G. Butenko, T. Pivneva and G. Skibo Long-term fate of grafted hippocampal neural progenitor cells following ischemic injury. Journal of Neuroscience Research 04/2014; https://doi.org/10.1002/jnr.23386.
• V. A. Shablii, M. D. Kuchma, V. M. Kyryk, H. M. Svitina, Yu. M. Shablii, L. L. Lukash, G. S. Lobintseva. Mesenchymal and trophoblast immunophenotype of multipotent stromal cells from human placenta. Biopolymers and Cell. 2014. Vol. 30. N 2. P. 118-121.
• Rybachuk O. A., Pivneva T. A. Prospects of the Use of Mesenchymal and Neuromesenchymal Stem Cells. Neurophisioligy. - 2013, Vol. 45, Issue 5-6, pp. 477-494.
• Kuchma, M.; Shablii, V.; Kyryk, V.; Onishchenko, A.; Lukash, L.; Lobintseva, G. Comparative phenotypic analysis of populations of hemotopoietic progenitor cells with different expression level of CD34 derived from placental tissue and umbilical cord blood. Bulletin of Taras Shevchenko National University of Kyiv. Vestn; 2013, 64(2):p18.
• Rybachuk О. А, Levin R. E., Кyryk V. М., Susarova D. K., Tsupykov О. M., Smozhanik E. G., Butenko G. M., Skibo G. G., Troshin P. A., Pivneva Т. А. Effect of a water soluble derivative of fullerene C60 on the features neural progenitor cells in vitro. Cell and organ transplantology. 2013; 1(1):102-107. 
• Kuchma M., Shablii V., Kyryk V., Onishchenko A., Shablii Yu., Lukash L., Lobintseva G. Phenotypic heterogenecity of hematopoietic progenitor cells from placental tissue: comarative analysis with umbilical cord blood and fetal liver. Cell and organ transplantology. 2013; 1(1):70-73. 
• Tsupykov O. M., Kyryk V. M., Rybachuk O. A., Poberezhnyi P. A., Mamchur A. A., Butenko H. M., Pivneva T. A., Skybo G. G. Effect of neural stem cell transplantation on cognitive functions of mice after cerebral ischemia-reperfusion. Cell and organ transplantology. 2013; 1(1):92-95. 
• V.A. Shablii, M.D. Kuchma, V.M. Kyryk, G.S. Lobyntseva Preservation of parenchymal and stromal progenitors in cryopreserved human fetal liver. Cytology and Genetics. 2012; 46(1):41-46.
• O.M. Tsupykov, A.O. Poddubna, K.G. Smozhanyk, V.M. Kyryk, O.V. Kuchuk, G.M. Butenko, E. Semenova, T.A. Pivneva, G.G. Skibo Integration of grafted neural progenitor cells in the host hippocampal circuitry after ischemic injury. Neurophysiology. 2011; 43(4):372-375.
• O.M. Tsupykov, T. A. Pivneva, A.O. Poddubna, V.M. Kyryk, O.V. Kuchuk, G.M. Butenko, G.G. Skibo Migration and Differentiation of Fetal Neural Progenitor Cells in the Brain of Ischemic Animals. Int.J.Phys.Pathophys. 2010; 1(40):25-34.

Conference abstracts

• Кирик ВМ, Устименко АМ, Цупиков ОМ. Сучасні підходи до комплексної оцінки маркерів клітинного старіння. Тези Всеукраїнської науково-практичної конференції з міжнародною участю "Тканинні реакції в нормі, експерименті, клініці-2025", Київ, 29-30.05.2025. Ukrainian scientific medical youth journal. 2025; 155 (2, Suppl. 2):82-83. https://doi.org/10.32345/SUPPLEMENT.2.2025.146-259
• Устименко АМ, Кирик ВМ, Іваніщев ВМ. Морфофункціональні характеристики мультипотентних мезенхімальних стромальних клітин жирової тканини в умовах змодельованої системної запальної відповіді у мишей. Тези Всеукраїнської науково-практичної конференції з міжнародною участю "Тканинні реакції в нормі, експерименті, клініці-2025", Київ, 29-30.05.2025. Ukrainian scientific medical youth journal. 2025; 155 (2, Suppl. 2):238-239. https://doi.org/10.32345/SUPPLEMENT.2.2025.146-259
• Кирик ВМ, Пархоменко О. М. Клітинне старіння в патогенезі серцево-судинних захворювань. Матеріали ХХVІ Національного конгресу кардіологів України, Київ, 24-27.09.2025 р. Український кардіологічний журнал. 2025; 32 (дод. 1):8-9. https://cardiohub.org.ua/wp-content/uploads/2024/09/Dodatok_1-UKZH-Tezy-2024.pdf
• Пархоменко ОМ, Кирик ВМ, Скаржевський ОА, Шумаков ОВ, Устименко АМ, Цупиков ОМ, Хомяков ДВ. Функція ендотелію та проліферативний потенціал імунних клітин крові як маркери серцево-судинного ризику у віддалені терміни після перенесеного інфаркту міокарда. Матеріали ХХVІ Національного конгресу кардіологів України, Київ, 24-27.09.2025 р. Український кардіологічний журнал. 2025; 32 (дод. 1):48. https://cardiohub.org.ua/wp-content/uploads/2024/09/Dodatok_1-UKZH-Tezy-2024.pdf     
• Кирик ВМ. Перспективи клітинної терапії запальних уражень міокарда. Матеріали ХХІV Національного конгресу кардіологів України, Київ, 19-22.09.2023 р. Український кардіологічний журнал. 2023; 30 (дод. 1): 152-153. https://cardiohub.org.ua/wp-content/uploads/2023/09/Dodatok_1_2023-Konhres-fin.pdf

• Кирик ВМ. Стовбурові клітини в регенерації міокарда. Тези Всеукраїнської науково-практичної конференції з міжнародною участю "Тканинні реакції в нормі, експерименті, клініці", Київ, 13-14.06.2024. Ukrainian scientific medical youth journal, 2024, 147 (Suppl. 2):49-50. https://doi.org/10.32345/SUPPLEMENT.2.2024.26-97

• Кирик ВМ., Чеботарьов М.Д. Нормативно-правове регулювання клітинної та тканинної терапії в Україні та світі. Матеріали ХХV Національного конгресу кардіологів України, Київ, 24-27.09.2024 р. Український кардіологічний журнал. 2024; 30 (дод. 1):8-9. https://cardiohub.org.ua/wp-content/uploads/2024/09/Dodatok_1-UKZH-Tezy-2024.pdf
• Устименко АМ, Кирик ВМ, Іваніщев ВМ. Морфофункціональні властивості культур мультипотентних мезенхімальних стромальних клітин жирової тканини у мишей різного віку. Матеріали ХХV Національного конгресу кардіологів України, Київ, 24-27.09.2024 р. Український кардіологічний журнал. 2024; 30 (дод. 1):20. https://cardiohub.org.ua/wp-content/uploads/2024/09/Dodatok_1-UKZH-Tezy-2024.pdf
• Лабунець ІФ, Пантелеймонова ТМ, Топорова ОК, Кирик ВМ, Чеботарьов МД, Літошенко ЗЛ. Ефекти мультипотентних мезенхімальних стромальних клітин пуповини людини при експериментальному паркінсонізмі в мишей із різним генотипом Н-2. Матеріали ХХV Національного конгресу кардіологів України, Київ, 24-27.09.2024 р. Український кардіологічний журнал. 2024; 30 (дод. 1):12-13. https://cardiohub.org.ua/wp-content/uploads/2024/09/Dodatok_1-UKZH-Tezy-2024.pdf
• Говбах ІО, Кирик ВМ, Устименко АМ, Булгакова НВ, Заводовський ДО, Соколовська І, Мазниченко АВ, Цупиков ОМ. Вплив трансплантації мультипотентних мезенхімальних стромальних клітин на скоротливість триголового м'яза литки мишей зі спадковою периферичною нейропатією. Тези доповідей Міжнародної конференції з нейронаук та Наукових читань з вісцеральній фізіології та патофізіології, присвяченої 100-річчю від дня народження академіка Платона Костюка, Київ, 19-21.11.2024. Фізіологічний журнал. 2024; 70(5):19-20. https://doi.org/10.15407/fz70.05S.001
• Кирик ВМ. Перспективи клітинної терапії запальних уражень міокарда. Матеріали ХХІV Національного конгресу кардіологів України, Київ, 19-22.09.2023 р. Український кардіологічний журнал. 2023; 30 (дод. 1): 152-153. https://cardiohub.org.ua/wp-content/uploads/2023/09/Dodatok_1_2023-Konhres-fin.pdf
• Kalmukova O, Kyryk V, Nepomnyaschy V, Savchuk O, Dzerzhynsky M. Exogenous melatonin action on different hypothalamic nuclei of obese rats: a chronotherapeutic approach. International Conference on Neurological Disorders andNeurorestoration, Dubrovnik, Croatia. Neurol Croat. 2022; 71 (Suppl. 2):49.
• Bukreieva TV, Nikulina VV, Skrypkina IYa, Kyryk VM, Vega AR, Chybisov OL, Duda OV, Shablii VA. Dynamic alterations of immunophenotype, C reactive protein and interleukine-6 levels in peripheral blood of severe COVID-19 patients. Biopolymers and Cell. 2021; 37(3):231
• Nikulina V, Kuchma M, Bukreieva T, Zahanich I, Kyryk V, Lobintseva G, Shablii V. Cryopreservation of placenta tissue allows isolating viable mesenchymal and hematopoietic stem cells, Cytotherapy, 2019. 21 (5), S78-S79
• Kalmukova OO, Yurchenko AV, Kyryk VM. Central serotonin and tryptophan levels in rats with diet-induced obesity at the different time of melatonin administration. Ukr. Biochem. J., 2018; 90(3):116.
• O. Kalmykova, V. Kyryk, M. Dzerzhinsky Morpho-functional state of serotoninergic neurons of hypothalamus arcuate nucleus of rats with diet-induced obesity under different times of melatonin administration.  “Modern aspects of biochemistry and cell biology”, 05-06 October 2017 Dnipro, Ukraine
• O. Kalmykova, O. Savchuk, M. Dzerzhinsky Melatonin improves skeletal muscles morphology and metabolism in high-calorie diet-induced obesity rat model. Third Kyiv Symposium “Smooth Muscle Physiology, Biophysiscs & Pharmacology: from genes and molecules to functions, disorders and their novel trearment opportunities”. 18-22 September 2017, Kyiv – Lutsk.
• Pokholenko Ia, Toporova O, Rymar S, Shuvalova N, Ignatchenko P, Moshynets O, Kyryk V, Dubey I, Kordium V. The development of functionalized scaffold for extracellular matrix replacement therapy of chronic wounds. Conference Abstracts "Innovative trends in genetic and regenerative medicine", Kyiv, November 9-10, 2017. Cell Organ Transpl. 2017; 5(2):233-234. https://doi.org/10.22494/cot.v5i2.79
• Kyryk VM. Advances in stem cells therapy for cardiovascular diseases. Cell and Organ Transplantology. 2017; 5(1):133. doi:10.22494/cot.v5i1.73.
• Shablii V., Areshkov P., Bukreieva T., Skrypkina I., Nikulina V., Dotsenko V., Kyryk V., Lobintseva G. Placenta-derived multipotent cells express syncytin-1 and posses ability to fuse with trophoblast. Placenta. 2017; 57:254. http://dx.doi.org/10.1016/j.placenta.2017.07.109
• Rybachuk OA, Arkhypchuk IV, Lazarenko YuA, Kyryk VM, Proshkina IO, Voitenko NV, Butenko GM. Development of the spinal cord injury model in mice. Conference Abstracts "Innovative trends in genetic and regenerative medicine", Kyiv, November 9-10, 2017. Cell Organ Transpl. 2017; 5(2): 236. https://doi.org/10.22494/cot.v5i2.79
• Rybachuk OA, Lazarenko YuA, Medvedev VV, Kyryk VM, Yaminsky YuYa, Voitenko NV, Tsymbaliuk VI. Cultivation of murine bone marrow derived stromal cells with NeuroGel^TM. Conference Abstracts "Innovative trends in genetic and regenerative medicine", Kyiv, November 9-10, 2017. Cell Organ Transpl. 2017; 5(2):236-237. https://doi.org/10.22494/cot.v5i2.79
• Koval L., Deryabina O., Ruban T., Ustymenko A., Kyryk V., Skok M. Mesenchymal stem cells express nicotinic acetylcholine receptors. Abstract book of International Symposium on Cell Biology jointly with 5th Ukrainian Congress for Cell Biology, Odessa, 2016.
• V Shablii, M Kuchma, H Svitina, I Skrypkina, P Areshkov, V Kyryk, Yu. Shablii, P. Klymenko, L. Lukash, G. Lobintseva Placenta-derived multipotent cells shared the expression of several trophoblast-related genes Placenta. 2016; 45:128.
• O. Toporova, I. Pokholenko, N. Shuvalova, V. Kyryk, O. Deriabina, P. Morgunov, D. Irodov, V. Kordium Genetic engineering of mesenchymal stem cells from umbilical cord Wharton’s jelly. Meeting Abstracts World Conference on Regenerative Medicine 2015, Leipzig, Germany, October 21-23.10.2015. Regenerative Medicine, 2015 Vol. 10 No. 07s, p. 220.
• O.K. Toporova, N.S. Shuvalova, V.M. Kyryk, S.M. Novikova, T.P., Ruban, P.V. Morgunov, Ia.O. Pokholenko, D.M. Irodov, V.A. Kordium Optimization of high-efficiency transfection of human mesenchymal stem cells with nanosized polyplexes. 3rd International research and practice conference Nanotechnology and Nanomaterials NANO-2015, August 26-29, 2015, Lviv:442.
• T.M. Lutsenko, V.M. Kyryk, A.M. Ustimenko, P.P. Klimenko, G.D. Koval Lactobacillus as a therapeutic agent in the endometriosis treatment. Abstract book of the International Conference for Young Scientists, September, 21-25, 2015, Kyiv, p. 171.
• V Shablii, H Svitina, M Kuchma, V Kyryk, I Skrypkina, P Areshkov Placental derived multipotent cells possess trophoblast specific features. Poster abstract book ISSCR 13th Annual Meeting, At Stockholm, Sweden, June 2015, P. 413.
• Kuchma M., Kyryk V., Svitina H., Shablii Yu., Lukash L., Lobintseva G., Shablii V. Hematopoietic stem/progenitor cells from human placental tissue, umbilical cord blood, and fetal liver. Poster abstract book ISSCR 13th Annual Meeting, At Stockholm, Sweden, June 2015, P. 227.
• H Svitina, Y Shablii, M Kuchma, V Kyryk, I Skrypkina, D Yevheniy, Transplantation of placental adherent multipotent cells does not affect mid/late tumor progression in dimethylhydrazine-induced colon carcinogenesis in rats. Poster abstract book ISSCR 13th Annual Meeting, At Stockholm, Sweden, June 2015, P. 17.
• Tsupykov O., Kyryk V., Butenko H., Skibo G. Neural stem cells transplantation promote the spatial learning recovery after ischemic brain injury. Abstracts of the 1st International Symposium on Mechanisms Underlying Memory Storage and Pathological Learning Montpellier, May 19, 2015, Abstract book, P. 29.
• O. Deryabina, N. Shuvalova, O. Toporova, I. Pokholenko, Y. Kasianenko, S. Verbovska, O. Pichkur, L. Pichkur, V. Kyryk, V. Kordium Combined cell-cytokine therapy for EAE treatment. ESGCT and NVGCT Collaborative Congress October 23-26, 2014. - Human Gene Therapy, 2014; 25 (11): A104.
• H Svitina, V Shablii, M Kuchma, V Kyryk, Y Shablii, I Skrypkina, L Garmanchuk, G Kyznezova, O Dzhus, E Denis, V Zhezhera, V Rubalchenko, G Lobintseva Trophoblast stem cells: characteristics and anticancer properties. 3^rd IPLASS Meeting, Spain, 10-12 September 2014 - p. 8.
• V Shablii, M Kuchma, V Kyryk, H Svitina, Y Shablii, I Skrypkina, L Lukash, G Lobintseva Multipotent trophoblast cells derived from native and cryopreserved human placental tissue. 3^rd IPLASS Meeting, Spain, 10-12 September 2014 - p. 7.
• M Kuchma, V Shablii, V Kyryk, H Svitina, Y Shablii, L Lukash, G Lobyntseva Characteristics of hematopoietic stem/progenitor cells from placental tissue, umbilical cord blood and fetal liver. 3^rd IPLASS Meeting, Spain, 10-12 September 2014. - p. 5.
• - Shablii V., Kuchma M., Kyryk V., Onishchenko G., Tsupykov O., Klymenko P., Kuchuk O., Gabrielyan A., Domansky T., Onishchenko V., Lukash L., Lobyntseva G. Characteristics of multipotent mesenchymal stromal stem cells derived from native and cryopreserved human placental tissue. "Журнал НАМН України". — 2012. — т. 18, додаток. — с. 184-185.
• Tsupykov O., Smozhanik E., Rybachuk O., Kyryk V., Kuchuk O., Patseva M., Butenko G., Skibo G., Pivneva T. Synapse formation between the grafted neural progenitor cells and host hippocampal neurons after ischemic injury. FENS Abstract, 2012, Volume 6, p048.25.
• V. Shablii, M. Kuchma, V. Kyryk et al. Characteristics of hematopoietic and mesenchymal stem cells isolated from cryopreserved human placental tissue. ISSCR, 10th Annual Meeting, Yokohama, Japan, June 13-16, 2012. P. 95.
• Volodymyr Shablii, Maria Kuchma, Vitaliy Kyryk, et al. Isolation and characterization of mutipotent mesenchymal stromal cells from native and cryopreserved human placental tissue. International Symposium on Cell Biology jointly with 3rd Ukrainian Congress for Cell Biology, Yalta, Ukraine, May 16 – 20, 2012. P.117.
• Maria Kuchma, Volodymyr Shablii, Vitaliy Kyryk, et al. Isolation and characterization of hematopoietic stem cells from native and cryopreserved human placental tissue. International Symposium on Cell Biology jointly with 3rd Ukrainian Congress for Cell Biology, Yalta, Ukraine, May 16 – 20, 2012. P.118.
• O. Kuchuk, O. Tsupykov, V. Kyryk Cultivation and osteogenic differentiation of murine bone marrow multipotent stromal cells in micromass culture. Abstracts of the DiSCUSS Meeting, December 5-8, 2011, Dresden, Germany. — P 43.
• O. Deryabina, O. Maslova, O. Sukhorada, V. Kyryk, V. Kordium Diversity of umbilical cord MSC and their variability during passaging ex vivo. Abstracts of the World Conference on Regenerative Medicine, November 2-4, 2011, Leipzig, Germany. — Regenerative Medicine. — 2011. — vol. 6, № 6, Suppl. 2. — P. 196. 
• O. Tsupykov, A. Poddubna, K. Smozhanyk, V. Kyryk, O. Kuchuk, T. Pivneva, G. Skibo, G. Butenko The grafted fetal neural progenitor cells can integrate in the adult hippocampal tissue after ischemic injury. Abstracts of the World Conference on Regenerative Medicine, November 2-4, 2011, Leipzig, Germany. — Regenerative Medicine. — 2011. — vol. 6, № 6, Suppl. 2. — P. 163. 
• M. Kuchma, V. Shablii, V. Kyryk, A. Onishchenko, G. Lobitseva Cryopreserved human placental tissue as sourse of hematopoietis and mesenchymal stem cells. World Cord Blood Congress III "Cord blood transplantation and immunobiology of haematopoietic stem cell transplant", Rome, 27-29 October, 2011. – P 171.
• V. Kyryk, O. Kuchuk, A. Rodnichenko, G. Butenko Proliferative activity of murine bone marrow cells after transplantation of mononuclear cells or sorted hematopoietic stem cells from bone marrow and fetal liver.   World Cord Blood Congress III "Cord blood transplantation and immunobiology of haematopoietic stem cell transplant", Rome, 27-29 October, 2011. – P 172. 
• O. Tsupykov, T. Pivneva, A. Poddubna, V. Kyryk, O. Kuchuk, G. Skibo Hippocampal-derived neural progenitor cells establish synaptic junctions after grafting into the adult ischemic brain. 8th IBRO World Congress of Neuroscience 2011 - Florence,  – D141.
• Tsupykov O.M., Poddubna A.O., Smozhanyk K.G., Kyryk V.M., Kuchuk O.V., Butenko G.M., Semenova E., Pivneva T.A., Skibo G.G. The integration of grafted neural progenitor cells in the host hippocampal circuitry after ischemic injury. V Congress of the Ukrainian Society for Neurosciences - Kyiv, June 6-10, 2011. – P. 44.
• Tsupykov O., Poddubna A., Ochenashko O., Petrenko A., Kyryk V., Kuchuk O., Butenko G., Skibo G., Pivneva T. Grafting of fetal neural progenitor cells into the adult ischemic brain. FENS Forum 2010, Amsterdam. FENS Abstr. - Vol.5, 016.31, 2010.
• Kuchuk O., Vasyliev R., Kyryk V., Butenko G. Mesenchymal stem cells and mouse embryonic fibroblasts potential for directed differentiation and 3D-cultivation. 8th International Stem Cell School in Regenerative Medicine. Scientific conference - Stockholm, 3-5 Sept 2010:27.
• Rodnichenko A., Kyryk V., Kuchuk O., Vasyliev R., Reznik O., Labunets I., Butenko G. The influence of stress on functional state of immune system and number of bone marrow haematopoietic stem cells and mesenchymal stromal cells of CBA/Ca and FVB mice. 8th International Stem Cell School in Regenerative Medicine. Scientific conference - Stockholm, 3-5 Sept 2010:53.
• Tsupykov O., Skibo G., Pivneva T., Kyryk V., Kuchuk O., Butenko G. Neural progenitor cells transplantation at experimental ischemic brain injury. 8th International Stem Cell School in Regenerative Medicine. Scientific conference - Stockholm, 3-5 Sept 2010:29.
• V.M. Kyryk, G.M. Butenko Effects of transplantation of bone marrow and fetal liver cells on cell subpopulations in murine thymus. World Conference on Regenerative Medicine, Leipzig, October 29-31, 2009. Regenerative Medicine. 2009. November 4 (6), Suppl. 2:4-5.
• Tsupykov O., Poddubna A., Kyryk V., Kuchuk O., Butenko G., Skibo G., Pivneva T. The fate of neural progenitor cells after transplantation into ischemic brain. Glia. 2009. 57 (Suppl. 13):74.